Orotic Acid Feeding
نویسنده
چکیده
1. The metabolism of 4-[4-14C]androstene-3,17-dione, 4-[4-14C]pregnene-3,20-dione, 5a-[4-14C]androstane-3a,17f8-diol, [4-4C]cholesterol, 7a-hydroxy4-6fi-3H]cholesten3-one, 5fi-[7fi-3H]cholestane-3a,7U-diol and [3H]lithocholic acid was studied in the microsomal fraction of livers from control and orotic acid-treated male rats. 2. As a result of the treatment the orotic acid-fed rats had fatty livers and subnormal concentrations of cholesterol and triglycerides in serum. 3. The 61)and 7a-hydroxylation of 4-androstene3,17-dione, the 2a-, 21)and 18-hydroxylation of 5a-androstane-3a,17fl-diol, and the 5a-reduction of 4-androstene-3,17-dione and 4-pregnene-3,20-dione were decreased by 40-50% in orotic acid-fed rats. Other oxidative and reductive reactions of the steroid hormones were not significantly affected. 4. The 12ax-hydroxylation of 7a-hydroxy4 cholesten-3-one was decreased by about 50%, whereas the 7r-hydroxylation ofcholesterol and the 26-hydroxylation of 50i-cholestane-3a,7a-diol were not significantly decreased. The 6fi-hydroxylation of lithocholic acid was stimulated by 40%. 5. The r.esults are discussed in relation to present knowledge of the hepatic drug-metabolizing enzymes and to the recent findings of an abnormal bile acid metabolism in liver disease.
منابع مشابه
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